This app allows you to calculate:

1. The expected coverage of amplicons in a sequencing run
2. The needed amount of sequencing to get a specific coverage
3. The depth of coverage given by a given by a specified amount of sequencing
4. The expected number of PCR amplicons given various conditions

This is to calculate the expected coverage of amplicons when running on the Oxford Nanopore (can also be used for Illumina)

Input read length currently nonfunctinal

Nanopore reported/estimated values

This app allows you to calculate the depth or data needed for WGS

Enter value for either Output Required or Depth Given on the side

Megabases of sequencing data

Coverage of genome

This app allows you to calculate the optimal PCR reaction

If you have nonspecific amplification, esitmate the effect size and adjust the input quantity accordingly