DNA Sequence Optimizer

Drop FASTA files here.

Files must be in FASTA format (.fasta or .fa)
Both DNA and amino acid sequences are supported.
Select target organism: 
Optimization method: 



 

DNA Sequence Optimization Pipeline Overview

This pipeline utilizes DNA Chisel, a powerful Python library for codon optimization and DNA sequence design.

  • FASTA sequence validation (DNA or amino acid sequences)
  • Amino acid to DNA conversion (if amino acid input)
  • Start codon verification (ATG added for amino acid sequences if missing)
  • Sequence length validation

  • Codon usage optimization
  • GC content adjustment
  • Translation verification
  • Performance optimization

  • Optimized sequence FASTA files
  • Detailed optimization report
  • GC content analysis
  • Email notification with results
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Amino Acid Sequence Processing

When processing amino acid sequences:

  • Start codons will be automatically handled during the DNA conversion process
  • All sequences will begin with ATG (Methionine) as required for proper translation
  • Original start codon settings are not applicable for amino acid sequences