Required
Input FASTA file:
No file chosen
Primer design region file:
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Enter email address:
Optional
Mispriming library FASTA file:
No file chosen
Desired melting temperature (Tm):
Output file name:
Desired primer length:
Min PCR product size:
Max PCR product size (primer design):
Max number of primer pairs to return:
Use NEB Q5 settings for primer3 (TRUE/FALSE):
Add Illumina partial adapters (TRUE/FALSE):
Require GC clamp:
Max length of mononucleotide repeat in primer:
